Work

Investigation of antibiofilm activity of bacteriophage ETEC-phage-TG and BC-VP against pathogenic Escherichia coli biofilms

Year: 2025

Type: article

Abstract: The aim of this study was to determine the antibiofilm activity of bacteriophages, ETEC-phage-TG and BC-VP, isolated from freshwater lakes against biofilm of Enterohemorrhagic Escherichia coli and Enteropathogenic Escherichia coli on polystyrene and stainless-steel surfaces. Bacteriophages isolated from previous research have the potential to degrade biofilm. Bacteriophage ETEC-Phage-TG have titer 4.50 × 10 5 PFU/mL towards EHEC and a Minimum Inhibitory Multiplicity of Infection of 0.01 (10 − 2 ). Bacteriophage BC-VP had titer 8.0877 × 10 4 PFU/mL towards EPEC and a Minimum Inhibitory Multiplicity of Infection of 0.001 (10 − 3 ). The bacteriophage ETEC-phage-TG was able to inhibit and destruct biofilm formation on polystyrene surface at 25.43% and 11.41% respectively. Bacteriophage ETEC-phage-TG was able to destruct biofilm formation (24 h and 48 h) on stainless steel surface at 37.87% and 39.21% respectively. Bacteriophage BC-VP was able to inhibit and destruct biofilm on polystyrene surface at 54.57% and 26.36% respectively, it was also able to destruct biofilm formation (24 h and 48 h) on stainless steel surface 33.21% and 43.94% respectively.

Source: BMC Research Notes

DOI: 10.1186/s13104-025-07432-8

Authors: King Archell, Javier Stuart, Diana Elizabeth Waturangi

Institution: Atma Jaya Catholic University of Indonesia

Language: English

Topic: Bacteriophages and microbial interactions

Subfield: Ecology

Field: Environmental Science

Domain: Physical Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $1,690