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Single-Molecule Vibrational Thermometry

Year: 2025
Type: article
Abstract: temperature measurements. Here, we describe Boltzmann-edge vibrational thermometry (BET) detected by anti-Stokes fluorescence, where the relative population of vibrationally excited molecules acts as a calibration-free reporter of local temperature based on the Boltzmann distribution. We demonstrate that BET microscopy is readily compatible with biological samples and achieves single-molecule sensitivity. We then show that local environments can be characterized through the modulation of vibrational temperature by mid-infrared absorption, allowing for BET fingerprinting. This work provides a foundation for sensitive vibrational thermometry in biological imaging.
DOI: 10.1021/acs.jpcb.5c04024
Authors: Philip A. Kocheril, Dongkwan Lee, Noor Naji, Rahuljeet S. Chadha, Ryan E. Leighton, Haomin Wang, Lu Wei
Language: English
Subfield: Biophysics
Open Access status: green
Funders: National Science Foundation, Alfred P. Sloan Foundation, Hertz Foundation, Heritage Medical Research Institute, National Institutes of Health, NIH Office of the Director, National Institute of General Medical Sciences, National Science Foundation Graduate Research Fellowship Program
Awards: G500318789, G5997576940, Graduate Research Fellowship Program (GRFP), G8215187426, G9000187807, Super-resolution Chemical Imaging for Biomedicine
OpenAlex ID: W4413140984
FWCI: 2.862
Cites: 71
Cited by: 8
Related to: 10