Generation of a human induced pluripotent stem cell line (LZUSHi003-A) from peripheral mononuclear cells of a patient carrying the c.3682delG mutation in the dystrophin gene
Year: 2025
Type: article
Abstract: Duchenne muscular dystrophy (DMD) is characterized by progressive muscle degeneration and is caused by mutations in the dystrophin gene. Here, we used Sendai virus to generate a human induced pluripotent stem cell line, LZUSHi003-A, from the peripheral mononuclear cells of a 13-year-old male patient carrying the DMD gene mutation c.3682delG. The LZUSHi003-A cell line retained this mutation, while expressing pluripotency markers. Additionally, it demonstrated a normal karyotype and could differentiate into three embryonic germ layers.
Source: Stem Cell Research
Authors: Jie Shi, Jiao Yang, Huijun Gao, Lan Yang, Yinpeng Zhang, Ansheng Zhao, Rui Yang, Zhonghong Xiong, Jing Yang, Bei Gao
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $2,360
Awards: G5432277401, G583378413
OpenAlex ID: W4413144557