小鼠背外侧被盖下核γ-氨基丁酸能神经元的全脑单突触输入
Year: 2025
Type: article
Abstract: The sublaterodorsal tegmental nucleus (SLD) is a critical hub for regulating rapid eye movement (REM) sleep and muscle atonia, with its dysfunction linked to various disorders such as REM sleep behavior disorder (RBD) and cataplexy. Despite its physiological significance, the presynaptic patterns influencing SLD γ-aminobutyric acid (GABA)ergic neurons-one of the primary neuronal subtypes within the SLD-remain poorly understood. This study applied a modified rabies virus tracing system combined with a Cre/loxP-based genetic approach to map and quantify the whole-brain monosynaptic afferents to SLD GABAergic neurons in mice. In total, 139 anatomically distinct nuclei were identified as sources of direct input, with predominant projections originating from the midbrain, pons, and medulla. Ipsilateral contributions accounted for 67.99% of all traced inputs, while 32.01% were contralateral. Prominent sources included the mesencephalic reticular nucleus, superior colliculus, oral part of the pontine reticular nucleus, gigantocellular reticular nucleus, lateral hypothalamic area, and zona incerta neurons. Several nuclei displayed contralateral projection biases. Immunofluorescence staining revealed molecular diversity among input neurons, suggesting that SLD GABAergic neurons integrate signals from anatomically and functionally distinct neuronal populations. These findings provide a comprehensive anatomical framework for understanding how SLD GABAergic neurons integrate multisource inputs and offer new perspectives for investigating their involvement in regulating complex physiological functions, including sleep and motor control.
Source: 动物学研究
Authors: Zhong Zhi-Gang, 复旦大学基础医学院神经生物学系老年基础医学研究平台, 上海 200032, Tang Shang-Qi, Ben Hui, Yang Jia-Lin, Chen Yong-Hua, Qu Wei-Min, Huang Zhi-Li, Qiu Mei-Hong, 复旦大学基础医学院药理学系医学神经生物学国家重点实验室, 教育部脑科学前沿科学中心, 脑科学研究院, 睡眠研究国际联合实验室, 上海 200032, 中国, 复旦大学附属中山医院麻醉科, 上海 200032, 中国, 复旦大学基础医学院生理学系, 上海 200032, 中国
Language: English
Subfield: Cognitive Neuroscience
Field: Neuroscience
Domain: Life Sciences
Open Access status: diamond
OpenAlex ID: W4413150899