Knee histology protocol for immunohistochemistry on cryosections v1
Year: 2024
Type: preprint
Abstract: Osteoarthritis (OA) is a chronic, degenerative condition characterized by whole-joint involvement, affecting the bone, cartilage, synovium, ligaments, and joint capsule. Given its widespread impact on millions of individuals, continued investigation into knee joint biology and pathology is critical. Here, we present a detailed protocol for the dissection, processing, cryosectioning, and immunohistochemical analysis of mouse knee joints. This method enables high-quality preservation of joint architecture and is optimized for the assessment of neural innervation using anti–βIII-tubulin, a pan-neuronal marker.
Language: English
Subfield: Rheumatology
Field: Medicine
Domain: Health Sciences
Open Access status: gold
OpenAlex ID: W4413154608