Precise copper ion release and recovery in polycaprolactone nanofiber scaffold: an antibacterial and osteogenic synergistic strategy for guided bone regeneration
Year: 2025
Type: article
Abstract: Introduction Infection control and bone regeneration remain major challenges in orthopedic therapy. To address these issues, we developed a multifunctional guided bone regeneration (GBR) nanofibrous material based on electrospun polycaprolactone (PCL). This material combines antibacterial and osteogenic properties using polydopamine (PDA) and copper ions (CuCu2+). Methods PCL nanofibers were produced via electrospinning, and Cu2+ ions were introduced through PDA-mediated surface modification to enable pH-responsive binding and controlled release. The material’s physicochemical properties were evaluated through structural analysis, mechanical testing, and release kinetics. Biological performance was tested using antibacterial assays and osteoblast (MC3T3-E1) cell cultures, including assessments of cell proliferation and key osteogenic gene expression (Runx2, Osx, ALP, OCN). Results The PCL-PDA-Cu composite showed strong structural integrity and mechanical stability. At a Cu2+ concentration of 0.1 M, it demonstrated: 1) strong antibacterial activity; 2) improved osteoblast proliferation; and 3) increased expression of osteogenic genes. The pH-dependent release system maintained effective Cu2+ levels while reducing cytotoxicity. Discussion By integrating PDA-mediated Cu2+ coordination with PCL nanofibers, we created a multifunctional platform that balances antimicrobial defense and bone regeneration. This controlled ion delivery strategy shows great promise for bone tissue engineering, especially in infection-prone environments.
Authors: Tongbin Liu, Akram Hassan, Matheel Zohair Yousif Alrawas, Caiyun Cui, Zaihan Ariffin
Language: English
Subfield: Biomedical Engineering
Field: Engineering
Domain: Physical Sciences
Open Access status: gold
Estimated APC paid: $3,801
OpenAlex ID: W4413180381