Work

Efficient Gene Knockdown in Adult Zebrafish Retina by Intravitreal Injection

Year: 2025

Type: article

Abstract: ) gene in adult zebrafish. We also describe how to collect and process retina samples for downstream immunohistochemistry, imaging, and quantification. Overall, this protocol will provide researchers with a straightforward, cheap, and effective method to perform targeted gene knockdowns in adult zebrafish retinas. Key features • This protocol provides researchers with an approach to knock down genes of interest in adult zebrafish retina without the use of electroporation. • This protocol can be performed without causing an acute damage response in the retina. • This protocol allows targeting of genes in both proliferating cells and terminally differentiated cells. • This protocol allows the retina to be collected and processed for further downstream analysis.

Source: BIO-PROTOCOL

DOI: 10.21769/bioprotoc.5436

Authors: Eyad Shihabeddin, Abirami Santhanam, Stephan Tetenborg, Alexandra Aronowitz, John O`Brien

Institutions: The University of Texas Health Science Center at Houston, Baylor College of Medicine, University of Houston

Language: English

Topic: Retinal Development and Disorders

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: diamond

Funders: National Institute of Mental Health, National Eye Institute