Work

Guanidinium-linked morpholino-siRNA chimera: synthesis, biophysical properties and in vitro activity

Year: 2025

Type: article

Abstract: This study introduces guanidinium morpholino RNA (GMO-RNA), an RNA analogue with enhanced cell permeability, nuclease resistance and RNA interference activity. Self-penetrating GMO-RNA chimeras leverage guanidinium-mediated endocytic uptake across multiple cell lines. Thermal studies validated duplex stability, and Eg5-targeting GMO-siRNA achieved effective gene silencing in cervical cancer cells, demonstrating therapeutic potential.

Source: Chemical Communications

DOI: 10.1039/d5cc03543k

Authors: Shalini Gupta, Atanu Ghosh, Subhamoy Pratihar, Maria Mukherjee, Jayanta Kundu, Surajit Sinha

Institution: Indian Association for the Cultivation of Science

Language: English

Topic: RNA Interference and Gene Delivery

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: bronze

Funder: Science and Engineering Research Board