Work

Isolation of Pyricularia and preparation of DNA for Illumina Sequencing v1

Year: 2025

Type: preprint

Abstract: This protocol describes procedures for isolating pure Pyricularia cultures from lesions in just 24 h and then generating DNA of sufficient purity for Illumina library production. The typical yield of purified DNA from 10 ml of culture is 5-10 µg.

DOI: 10.17504/protocols.io.14egnrb7pl5d/v1

Author: Mark Farman

Institution: University of Kentucky

Language: English

Topic: Plant Pathogens and Fungal Diseases

Subfield: Cell Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: gold

OpenAlex ID: W4413202181

Cites:

Cited by: 1

Related to: 10