Reaction pathways and technologies of in vitro DNA synthesis
Year: 2025
Type: article
Abstract: Since DNA is the main carrier of genetic information, in vitro DNA synthesis plays a key role in the research of life science. Through the systematic research of DNA synthesis, the transformation or innovation of organisms could be controlled. This review has summarized the detailed synthesis reaction pathways that are classified by the key materials, including phosphodiester, phosphotriester, phosphite triester, phosphoramidite, and various enzymes. The latest advances of different fabrication technologies such as inkjet printing, on-column synthesis, photochemistry, and electrochemistry for in vitro DNA synthesis have also been investigated, especially inkjet-printing methods. Then, the developing trend of reaction routes and technologies for in vitro DNA synthesis has been proposed.
Source: Cell Reports Physical Science
Authors: Haihua Zhou, Huizeng Li, Zhimei Jia, Saisai Niu, Yanlin Song
Institutions: Chinese Academy of Sciences, Institute of Chemistry
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $5,620
OpenAlex ID: W4413218240