Work

Aptamer-functionalized magnetic covalent organic frameworks for rapid extraction and LC-MS/MS quantification of gentamicin in milk

Year: 2025

Type: article

Abstract: Gentamicin (GEN) residues in food products, arising from its extensive use in veterinary medicine, have prompted stringent regulations and a demand for simple, rapid detection methods. In this study, aptamer-modified magnetic covalent organic frameworks (COFs), denoted as TpDBD@Fe₃O₄@apt, were synthesized and employed as adsorbents in magnetic solid-phase extraction (MSPE) for the highly specific capture and enrichment of GEN residues in milk. Several factors influencing MSPE efficiency were systematically evaluated and optimized. Coupled with liquid chromatography-tandem mass spectrometry (LC-MS/MS), the developed method exhibited good linearity within the linear range (R 2 ≥ 0.999), low detection limits (0.48–0.54 ng/mL), satisfactory recoveries (92.7 %–108.8 %), and high precision (RSDs ≤10.8 %). The proposed method was successfully applied to real milk samples, providing an efficient solution for the detection of GEN residues.

Source: Food Chemistry

DOI: 10.1016/j.foodchem.2025.145889

Authors: Hongmei Liu, Hao Cao, Yangjiayi Xiang, Cong Hu, Gou Wu, Yan Li

Institutions: Fudan University, Pudong Medical Center, Humanwell Healthcare Group (China), Shanghai Medical College of Fudan University

Language: English

Topic: Analytical chemistry methods development

Subfield: Analytical Chemistry

Field: Chemistry

Domain: Physical Sciences

Open Access status: hybrid

Estimated APC paid: $4,930

Funders: National Natural Science Foundation of China, Science and Technology Commission of Shanghai Municipality