Cellular Analysis of the Scs7 Knockout Strain Under 5-hydroxymethylfurfural Stress in Saccharomyces Cerevisiae
Year: 2025
Type: article
Abstract: 5-hydroxymethylfurfural (HMF) is a recognized indicator of non-enzymatic browning and deterioration produced by excessive heating or storage of foods, but HMF can be converted by sulfotransferase (SULT) into 5-sulfooxymethylfurfural (SMF), a compound that harms human skeletal muscle, kidneys and other organs.Therefore, exploring the underlying HMF tolerance mechanisms in organisms is important for in protecting the human body from the harm.In our previous studies that the candidate genes GUP1, SCS7, LAS21 and ORM1 in the membrane fluidity pathway may be associated with HMF tolerance in S. cerevisiae.In this study, based on the spot-plate test, it was found that the SCS7 gene knockout strain was found to be sensitive in 60 mM HMF.According to the subcellular structure observation, after 3 h of HMF treatment, the proportion of cells with endoplasmic reticulum (ER) damage, mitochondrial damage, and reactive oxygen species (ROS) accumulation in the SCS7 gene knockout strain increased by 64.61%, 11.68%, and 15.87%, respectively, compared with the standard strain BY4741.In summary, gene SCS7 promotes the stability of ER and mitochondria structure and the clearance of ROS in cells, providing guidance for the protection and treatment of organisms exposed to HMF.
Authors: X.M. XIAO, B.R. MOU, H.Y. WANG, F.J. LU, Q. LI, L. ZHAO, C. FU, W.C. LONG, J.F. LAI, C.H. LI, W. WU, M.G. MA, Y.J. YANG
Institutions: Leshan Normal University, Sichuan Agricultural University
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: diamond
OpenAlex ID: W4413239387