Work

Heterodimeric protein entangling motifs: systematic discovery, feature analysis, and topology engineering

Year: 2025

Type: article

Abstract: , stability enhancement crucial for executing functions like regulation and catalysis). Six premium motifs were selected for experimental validation. Upon ring closure mediated by orthogonal split inteins, all six motifs led to protein hetero[2]catenanes with varying efficiency, providing versatile templates for making mechanically interlocked protein conjugates, such as Förster resonance energy transfer pairs and bispecific binders. The study not only helps untangle the influence of chain entanglements on protein properties but also provides a modular platform to enrich the toolbox of protein topology engineering.

Source: Chemical Science

DOI: 10.1039/d5sc03953c

Authors: Lianjie Xu, Xibao Tian, Wen-Bin Zhang

Institutions: Peking University, Beijing National Laboratory for Molecular Sciences

Language: English

Topic: Biochemical and Structural Characterization

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: diamond

Funders: National Natural Science Foundation of China, Peking University, National Key Research and Development Program of China, Beijing National Laboratory for Molecular Sciences