Work

Temporal and spatial evolution of transparency in tissue clearing

Year: 2025

Type: article

Abstract: Tissue clearing is done to enable detecting signals from fluorescent labels deep within a specimen, permitting three-dimensional imaging of structures within these specimens. This study explores the temporal and spatial evolution of tissue transparency during the clearing process, focusing on the diffusion mechanisms of optical clearing agents, such as benzyl alcohol benzyl benzoate. Axial and radial configurations were studied, with both approaches yielding comparable results. The radial configuration enables simpler exponential fitting, while the axial configuration provides deeper insights through simulations, a key observation being that tissue edges clear faster than the core. Experimental measurements on five tissue types (chicken breast, beef, pork loin, lamb chop, and pig brain) yielded a diffusion constant of 2.7 ± 0.6×10−6 cm2/s.

Source: Biophysics Reviews

DOI: 10.1063/5.0267502

Authors: L. Dong, G. W. Luiten, E. van der Giessen, P. J. van der Zaag

Institutions: University of Groningen, University Medical Center Groningen

Language: English

Topic: Advanced Fluorescence Microscopy Techniques

Subfield: Biophysics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: hybrid

Funder: China Scholarship Council

Award: G6562617273