Protective Effects of Methanol and Aqueous Leaf Extracts of Annona muricata Linn on Sodium Selenite-Induced Oxidative Stress on the Liver in Selenite Cataract Rat Model
Year: 2025
Type: article
Abstract: Background and objective: Oxidative stress induces lens opacity.Annona muricata extracts have demonstrated potential anti-oxidant and medicinal efficacy.This study evaluated the protective effects of methanol and aqueous leaf extracts of Annona muricata Linn on sodium selenite-induced oxidative stress on the liver in a selenite cataract rat model Methods and study design: The study involved 55 Wistar suckling pups randomly split into 11 groups.Sodium selenite (30 μmol/kg, S.C) was administered on the 10th day of life, with the exception of the normal control group.Group C (positive control) was treated with the reference drugs (vitamin C) (100 mg/kg, oral) for 21 days.Groups D, E, F and G received methanol extract orally at 100 mg/kg, 150 mg/kg, 200 mg/kg, and 250 mg/kg, respectively for 21 days.Groups H, I, J and K received aqueous extract orally at 100 mg/kg, 150 mg/kg, 200 mg/kg, and 250 mg/kg, respectively, for 21 days.Water was given to group A (normal control) instead of the extract and no treatment was given to group B (negative control).Results: Compared to test control, the malondialdehyde levels of the other groups, and the catalase activity of the aqueous extract treated groups were significantly (p<0.05)lower, and the glutathione peroxidase activity of the aqueous extract treated groups were significantly (p<0.05)higher.The aqueous extract treatment also significantly lowered plasma alanine transaminase (ALT) and aspartate transaminase (AST) activities (p<0.05).Histopathology revealed mild inflammatory cells in groups treated with Methanol extract but showed normal hepatocytes in groups treated with aqueous extract.Conclusion: These findings show that the methanol and aqueous extracts of Annona muricata have dosedependent hepatoprotective properties and may be a viable plant for the development of an anti-hepatotoxic drugs.
Authors: Nkechi Ujunwa Maduagwu, Chukwuebuka Rufus Maduagwu, Michael Chinonso Okike, Nnamdi Hyacinth Nnubia, Damian Chibueze Onah, Wisdom Kelechukwu Emmanuel, Ndukwe Chika Kalu, Precious Chinonso Ugwuoke, Ugwuanyi Amuche Louis, Igwe Cynthia Amarachukwu, Onyeaghala Santus Uchendu, Abraham-Ajala Eunice, Ellis Ehizojie Momoh, Ikenna Kingsley Uchendu
Language: English
Subfield: Biochemistry
Domain: Life Sciences
Open Access status: hybrid
OpenAlex ID: W4413249495