Figure 4 from One-Carbon Metabolism Inhibition Depletes Purines and Results in Profound and Prolonged Ewing Sarcoma Growth Suppression
Year: 2025
Type: other
Abstract: Pharmacologic inhibition of one-carbon metabolism results in inhibition of Ewing sarcoma spheroid growth and colony formation, resulting in G1 phase to early S-phase arrest. A, Analysis of the inhibitory effect of SHIN1 and PRTX on the growth of SK-ES-1 spheroids. *, P < 0.001. B, Colony-forming assay of SK-ES-1 and TC-71 cells treated with SHIN1 (10 µmol/L), AGF347 (10 µmol/L), and PRTX (1 nmol/L) for 6 days and then grown without drugs for seven additional days. Quantitative results are shown in the graph to the right. *, P < 0.0001. C, Cell-cycle analysis of SK-ES-1 and TC-71 cells treated with the indicated compounds for 48 hours. *, P < 0.01 from replicate experiments.
Authors: Sara Zirpoli, Noah Copperman, Shrey Patel, Alexander Forrest, Zhanjun Hou, Larry H. Matherly, David M. Loeb, Antonio Di Cristofano
Language: English
Subfield: Pulmonary and Respiratory Medicine
Field: Medicine
Domain: Health Sciences
Open Access status: gold
OpenAlex ID: W4413253277