Biosynthesis of Antituberculosis Antibiotic Capreomycin Involves a trans-Iterative Adenylation Domain within the Nonribosomal Peptide Synthetase Machinery
Year: 2025
Type: article
Abstract: High Resolution Image Download MS PowerPoint Slide Capreomycin (CMN) is a nonribosomal peptide (NRP) antituberculosis antibiotic. CMN biosynthesis involves a non-canonical trans -iterative adenylation (A) domain. Here, we report that the A domain-less nonribosomal peptide synthetase (NRPS) module CmnI utilizes another module’s A domain CmnA-A 1 to load the required amino acid onto its thiolation (T) domain. This study provides evidence of an unusual mode of NRP biosynthesis in bacteria, involving a trans -iterative A domain in the NRPS machinery.
Source: Organic Letters
Authors: Yi-Ting Lai, Chun-Yu Peng, Hsiao-Tzu Liao, Po-Yun Hsiao, Chang-Ko Hsieh, Yu-Ru Luo, Sheng-Cih Huang, Yung-Lin Wang, Thomas Ma, Ya-Rong Chen, You-Min Kuo, Yen-Cheng Lin, John Chu, Chin-Yuan Chang
Language: English
Subfield: Pharmacology
Field: Medicine
Domain: Health Sciences
Open Access status: hybrid
Estimated APC paid: $4,500
Awards: G2298007856, G4984045863, G892990847
OpenAlex ID: W4413257587