Figure 2 from PSA Secretion from Single Circulating Tumor Cells of Metastatic Castration-Naïve Prostate Cancer Patients
Year: 2025
Type: other
Abstract: Workflow schematically illustrating the DLA processing to obtain viable CTCs. (1) DLA enrichment using the reduced enrichment reagent (RER) protocol; (2) staining using anti–PSMA-PE, anti–CD45-APC, and viability dye calcein AM; (3) sorting of calcein+ and CD45– CTCs enriched from the DLA; (4) seeding into the VyCAP nanowells; (5) imaging using a fluorescence inverted scanning microscope to visualize cells captured in the nanowells; (6) PSA secretion from single cells and PSA capture overnight (24 hours) using an anti–PSA-coated membrane and addition of anti–IgG-PE to visualize the imprint; (7) staining of the membrane for visualization of PSA secretion spots; and (8) correlation of PSA spots with cells with the help of imprint and the SPOT software to identify PSA-secreting and non-secreting PSMA+/PSMA− CTCs.
Authors: Eshwari Dathathri, Fikri Abali, Michiel Stevens, Richell Booijink, Tanja C. van Dijk, Khrystany T. Isebia, John W.M. Martens, Jaco Kraan, Nick Beije, Martijn P. Lolkema, Peter A.W. te Boekhorst, Paul Hamberg, Brigitte C.M. Haberkorn, Danny Houtsma, Joan van den Bosch, Wendy M. van der Deure, Ruchi Bansal, Leon W.M.M. Terstappen
Language: English
Subfield: Pulmonary and Respiratory Medicine
Field: Medicine
Domain: Health Sciences
Open Access status: gold
OpenAlex ID: W4413293028