Figure S3 from PSA Secretion from Single Circulating Tumor Cells of Metastatic Castration-Naïve Prostate Cancer Patients
Year: 2025
Type: other
Abstract: Visualization of the nanowells and membrane using the VyCAP SPOT software (A) Imprint of IgG antibody printed onto a PVDF membrane using a nanowell array, is used to align and match the secreted proteins to the corresponding cell inside a well. (B) The PSA proteins secreted by the individual CTC is printed onto PVDF membrane. (C) A brightfield image of the nanowell arrays filled with CTC. (D)Viable CTC inside individual wells of the chip. (E) PSMA-PE expression of the CTC inside the nanowells. (F) CD45-APC expression of the CTC inside the chip. Images in the lower left corner illustrate a small part of the membrane showing the IgG spots (A) and PSA spots (B) with their corresponding well-id analyzed in the VyCAP SPOT software. The corresponding CTC secreting PSA can now be located inside the nanowell array (C-F) to determine cell phenotype and isolated for downstream analysis.
Authors: Eshwari Dathathri, Fikri Abali, Michiel Stevens, Richell Booijink, Tanja C. van Dijk, Khrystany T. Isebia, John W.M. Martens, Jaco Kraan, Nick Beije, Martijn P. Lolkema, Peter A.W. te Boekhorst, Paul Hamberg, Brigitte C.M. Haberkorn, Danny Houtsma, Joan van den Bosch, Wendy M. van der Deure, Ruchi Bansal, Leon W.M.M. Terstappen
Language: English
Subfield: Pulmonary and Respiratory Medicine
Field: Medicine
Domain: Health Sciences
Open Access status: gold
OpenAlex ID: W4413293072