A 19F‐ATCUN Peptide NMR Probe for Selective Cu2+ or Ni2+ Sensing in Complex Biological Media
Year: 2025
Type: article
Abstract: We report the design and synthesis of ratiometric 19F‐ATCUN (Xxx‐Zzz‐His) peptide probes for the selective and reversible detection of Ni2⁺ and Cu2⁺ by 19F NMR spectroscopy. In contrast to fluorescence‐based approaches, 19F NMR enables background‐free detection in biological media, due to the absence of fluorine in native biomolecules. The probes remain stable and functional in complex cell media (DMEM), enabling the detection of Cu2⁺ and Ni2⁺ by 19F NMR with a limit of detection (LOD) of 4 µM and quantification (LOQ) of 10 µM, relevant for studying copper‐related pathologies.
Source: Chemistry - A European Journal
Authors: Marie‐Julie Tilly, Enrico Falcone, Bruno Kieffer, Yannis‐Nicolas François, Peter Faller, Marine Desage‐El Murr, Laurent Raibaut
Language: English
Subfield: Spectroscopy
Field: Chemistry
Domain: Physical Sciences
Open Access status: hybrid
Estimated APC paid: $4,640
Funder: China Scholarship Council
OpenAlex ID: W4413307713