Work

A ratiometric fluorescent probe monitoring lipid peroxidation within lipid droplets in foam cells

Year: 2025

Type: article

Abstract: Lipid peroxidation (LPO) in foam cells is crucial for regulating atherosclerosis progression. It correlates with lipid uptake and the state of lipid droplets. In this study, we report a lipid droplet-targeted fluorescent LPO probe, Ld-LPO. It selectively responds to LPO, resulting in a significant fluorescence shift from 590 to 525 nm, enabling a ratiometric imaging of LPO in lipid droplets. Ld-LPO traces lipid droplets in foam cells, revealing a correlation between LPO and lysosomal engulfment. We found that lipid droplets engulfed by lysosomes exhibit higher LPO, attributed to low-density lipoprotein accumulation in lysosomes. Furthermore, Ld-LPO is compatible with dual-color flow cytometry, facilitating high-throughput analysis of LPO in foam cells.

Source: Smart Molecules

DOI: 10.1002/smo2.70015

Authors: Dingxuan Li, Caiwei Lu, Shulan Sun, Xiaoxi Li, Yi Xiao, Xinfu Zhang

Institutions: Dalian University of Technology, State Key Laboratory of Fine Chemicals, Liaoning Cancer Hospital & Institute

Language: English

Topic: Molecular Sensors and Ion Detection

Subfield: Spectroscopy

Field: Chemistry

Domain: Physical Sciences

Open Access status: diamond

Funders: National Natural Science Foundation of China, Fundamental Research Funds for the Central Universities