Work

Regulatory role of LncRNA FMR1-AS1 in the pathogenesis of alzheimer’s disease based on bioinformatics and in vitro experimental validation

Year: 2025

Type: article

Abstract: Alzheimer's disease (AD) is a major cause of dementia, characterized by β-amyloid (Aβ) plaque accumulation and Tau protein hyperphosphorylation. Although long non-coding RNAs (lncRNAs) have been implicated in neurodegenerative diseases, their roles in AD remain unclear. This study analyzed RNA sequencing data from the brain tissues of 17 AD patients and 19 healthy controls (GEO: GSE138260) to construct a gene co-expression network and identified eight lncRNAs strongly associated with AD. FMR1-AS1 was selected for functional validation. In an Aβ1-42-induced SH-SY5Y neuronal injury model, overexpression of FMR1-AS1 significantly increased cell viability ([Formula: see text]), inhibited apoptosis ([Formula: see text]), and reduced Tau hyperphosphorylation ([Formula: see text]). FMR1-AS1 also alleviated oxidative stress by lowering reactive oxygen species (ROS) levels ([Formula: see text]), enhanced superoxide dismutase (SOD) activity ([Formula: see text]), and decreased malondialdehyde (MDA) content ([Formula: see text]). Knockdown of FMR1-AS1 exacerbated neuronal damage. These results demonstrate that FMR1-AS1 exerts neuroprotective effects by regulating apoptosis, oxidative stress, and Tau pathology. The study highlights FMR1-AS1 as a potential therapeutic target for AD and may advance the understanding of lncRNA-mediated regulatory mechanisms in neurodegeneration.

Source: Scientific Reports

DOI: 10.1038/s41598-025-15242-y

Authors: Xiang Yu, Hao Cai, Cai He, Zhong Ouyang, Yongchang Li, Liang Chen

Institutions: Hunan Normal University, Hunan Provincial People's Hospital, Shantou University

Language: English

Topic: Alzheimer's disease research and treatments

Subfield: Physiology

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $2,690