Year: 2025
Type: preprint
Abstract: This protocol describes in vitro methods for evaluating phagocytosis using the macrophage-like cell line, RAW 264.7. The two methods discussed here include (1) a photographic phagocytosis assay, which is compatible with any target that is visible via 100x microscopy, and measures total uptake of target material per macrophage, and (2) the phagocytosis-kill assay, which measures killing of phagocytosed microbial targets by the macrophages.
Authors: Matthew Slarve, travis nielsen, Dongran Yu, Jordyn VanOrman, Brian Luna, Brad spellberg
Institution: University of Southern California
Language: English
Subfield: Immunology
Field: Immunology and Microbiology
Domain: Life Sciences
Open Access status: gold
OpenAlex ID: W4413318673