Work

Investigating a new Dillenia ovata endophytic bacteria to produce antioxidants and anti-diabetes activity in vitro and in vivo

Year: 2025

Type: article

Abstract: A novel endophytic bacterium, Bacillus sp. DO-R5, with strong antioxidant and anti-diabetic properties, was isolated from Dillenia ovata , a traditional Vietnamese medicinal plant. Bacillus sp. DO-R5 was identified using morphological characteristics, SEM, and 16S rRNA sequencing, demonstrated the highest bioactivity among the 19 isolates. Optimal fermentation conditions (8.9 g/L D-glucose, pH 7.5, 38 °C, and 70.5 h) were determined using the Box-Behnken design. Under these conditions, Bacillus sp. DO-R5 significantly enhanced the production of bioactive compounds including diosmin, rutin, and chlorogenic acid, and exhibited improved in vitro antioxidant, anti-inflammatory, and anti-diabetic activities. In vivo , the extract effectively reduced blood glucose, regulated lipid profiles, supported weight control, and protected organs. These findings emphasize the potential therapeutic use of D. ovata endophytic bacteria as a natural source of anti-diabetic drugs.

Source: Biotechnology Reports

DOI: 10.1016/j.btre.2025.e00921

Authors: Linh Chi Tran, Chong Kim Thien Duc, Tuan Trong Nguyen, Duy Toan Pham, Danh Thai Luu, Trang Thi Xuan Dai

Institutions: Can Tho University, Nam Can Tho University

Language: English

Topic: Microbial Natural Products and Biosynthesis

Subfield: Pharmacology

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $3,480