Expanded application to plant reproductive tissues of a branched DNA probe‐based in situ hybridization method
Year: 2025
Type: article
Abstract: Premise Detecting clear tissue‐ and organ‐specific patterns of gene expression is key to understanding the genetic mechanisms that control plant development. In situ hybridization (ISH) of mRNA is one of the most precise, yet most challenging approaches to gene expression assays. Methods and Results Detection of histone H4 expression in reproductive tissues of Mimulus lewisii , a model angiosperm, was optimized using the RNAscope ISH assay. The optimized protocol was used to detect histone H4 expression in reproductive tissues of two gymnosperm species, Taxodium distichum and Juniperus virginiana , without further need for species‐specific optimization. Additionally, the optimized protocol was used to detect expression of CYCLOIDEA transcription factors in M. lewisii reproductive tissues without further optimization and with results similar to those previously reported. Conclusions The RNAscope assay can quickly and sensitively generate high‐quality ISH results in reproductive tissues across a breadth of plant species.
Source: Applications in Plant Sciences
DOI: 10.1002/aps3.70020
Institution: University of Kansas
Language: English
Subfield: Plant Science
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $2,340
Awards: MRI: Acquisition of a High-Performance Computing Cluster for Science and Engineering Research at the University of Kansas, G2853296776, G2921881625, Collaborative Research: EDGE CMT: Linking genomic variation to complex floral trait adaptation across a species boundary, G2972030790, RaMP: Making meaningful connections: Fostering the Integration of Biodiversity Patterns with Genetic Evolutionary Mechanisms (BioGEM), Graduate Research Fellowship Program (GRFP), G7285114270, CAREER: A phylogeny of living and extinct confers, G9000108934, G9000150948, Kansas IDeA Network of Biomedical Research Excellence 5
OpenAlex ID: W4413358009