Generation of human embryonic stem cell line expressing dCas9-TET1 fusion protein for epigenetic editing
Year: 2025
Type: article
Abstract: CRISPR-based epigenome editing systems can induce site-specific transcriptional activation or repression of target genes. Ten-eleven translocation methylcytosine dioxygenase 1 (TET1) is a transcriptional activation effector involved in the cytosine demethylation of CpG dinucleotides in gene regulatory regions. In this study, we generated a human embryonic stem cell line that stably expresses catalytically dead Cas9 (dCas9) fused to the catalytic domain of TET1 via lentiviral transduction. This cell line can be used for locus-specific transcriptional activation in combination with single guide RNAs and serves as a valuable tool for epigenetic regulation in stem cell and organoid models.
Source: Stem Cell Research
Authors: Ji-Woo Kim, Seongyea Jo, Eun-Hye Kang, Ji Hyeon Ryu, Haneul Noh, Han-Jin Park, Hyemin Kim
Institution: Korea Institute of Toxicology
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $2,360
Awards: G4397886443, G7452130367
OpenAlex ID: W4413366786