Multi-parametric magnetic resonance imaging evaluation of a novel ex ovo chick chorioallantoic membrane model for cancer research
Year: 2025
Type: article
Abstract: Background: pancreatic cancer CAM model. Furthermore, this study explored the potential of functional MRI techniques in quantitatively discriminating pathological characteristics. Methods: culture and subsequent experiments. On ED7, cancer cell mixtures [pancreatic cancer cells mixed with Matrigel and Dulbecco's modified Eagle medium (DMEM)] were implanted on the CAM to establish the cancer model. At each observation time point (ED10, ED12, and ED14), eight models were randomly selected for multi-parametric MRI (mpMRI) via a 3.0 Tesla (T) magnetic resonance (MR) scanner and pathological examination. The remaining models were observed on ED16. Quantitative assessments including tumor nodule volume, apparent diffusion coefficient (ADC), and T1 values were further correlated with histopathological findings. Results: ms on ED16, with no significant variations (P=0.69 for all intergroup comparisons). Pathological examination confirmed viable tumor cells in all specimens, demonstrating ring-like tumor tissue by ED12 that progressively replaced the Matrigel over subsequent time points. Conclusions: CAM serves as a reliable and reproducible experimental platform. Conventional 3.0 T MRI provides high-resolution morphological assessment of tumor dynamics, whereas functional imaging techniques offer promising capabilities for quantifying inter-nodular heterogeneity.
Authors: Guodong Feng, Weili Peng, Zhenfeng Ma, Houhui Shi, Jiaqi Zhang, Bin Song, Jun Chen, Yi-Jun Liang
Language: English
Topic: MRI in cancer diagnosis
Subfield: Radiology, Nuclear Medicine and Imaging
Field: Medicine
Domain: Health Sciences
Open Access status: diamond
OpenAlex ID: W4413369204