Work

Protocol for the colocalization of yeast peroxisomal membrane proteins and their binding partners using stimulated emission depletion microscopy

Year: 2025

Type: article

Abstract: Peroxisomes are highly dynamic organelles that play crucial roles in cellular metabolism. Here, we present a protocol to investigate peroxisomal membrane proteins and their binding partners in the yeast Hansenula polymorpha using stimulated emission depletion (STED) nanoscopy. We describe steps for strain construction to facilitate specific STED-compatible labeling, automated STED imaging, and semi-automated data analysis. This protocol enables examination of the dynamic alterations in the colocalization of the peroxisomal membrane protein Pex3 and its binding partner Atg30. For complete details on the use and execution of this protocol, please refer to de Lange et al. 1

Source: STAR Protocols

DOI: 10.1016/j.xpro.2025.103998

Authors: Frank N. Mol, Eline M.F. de Lange, Ida J. van der Klei, Rifka Vlijm

Institutions: University of Groningen, Zernike Institute for Advanced Materials

Language: English

Topic: Advanced Fluorescence Microscopy Techniques

Subfield: Biophysics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: gold

Estimated APC paid: $1,550

Funder: Nederlandse Organisatie voor Wetenschappelijk Onderzoek