Year: 2025
Type: article
Abstract: The establishment of the vertebrate body plan is orchestrated by the organizer, a specialized group of cells with inductive properties that guide axial specification during early development. In avian embryos, organizer cells reside within Hensen’s node, a transient structure located at the tip of the primitive streak. Despite its pivotal role during gastrulation, the cellular architecture of the Hensen’s node remains poorly understood. Here, we show that Hensen’s node is composed of two transcriptionally and functionally distinct organizer populations. In addition to anterior GSC-expressing cells associated with head induction, we identify a posterior population co-expressing organizer and mesodermal genes. These posterior cells exhibit trunk-inducing activity when transplanted into naïve tissue. Our findings reveal that the organizer is a dynamic and spatially compartmentalized structure, and that temporal changes in the relative abundance of anterior and posterior populations underlie shifts in its inductive capacity, ensuring coordinated patterning along the body axis. The organizer is a signaling center in the embryo that orchestrates the formation of body axes. Here they show that Hensen’s Node contains two main cell types and that changes in their abundance alter its ability to induce head versus trunk structures.
Source: Nature Communications
Institutions: Boston Children's Hospital, Harvard University, Cornell University
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $6,990
OpenAlex ID: W4413438962