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Identification of gene targets regulated by the IclR-like regulator SL1344_3500 in Salmonella Typhimurium

Year: 2025
Type: article
Abstract: ABSTRACT Transcriptional regulation of metabolic operons is important for optimal carbohydrate use and for mitigating the accumulation of toxic intermediates. Here, we characterize SL1344_3500 , encoding a putative IclR-like regulator in Salmonella enterica Typhimurium. We present genetic and transcriptional evidence that it regulates the expression of two neighboring operons, one designated here as xynABC , enables utilization of xylonate as a sole carbon source. Furthermore, our findings indicate that SL1344_3500 is important for luminal growth in several mouse models, exerting its effects through the suppression of the xynABC operon. Based on the observation that the Δ SL1344_3500 deletion can be stably complemented in vivo , we developed a plasmid stabilization strategy. This gene complementation approach shows promise for generating stable gene reporters for long-term colonization experiments. IMPORTANCE Understanding transcriptional regulation in Salmonella enterica Typhimurium is crucial for revealing how enteric pathogens optimize metabolism to compete with commensals in the gut. SL1344_3500, an IclR-like transcriptional regulator controlling genes linked to sugar acid metabolism, is essential for luminal growth in mouse models through gene suppression and represents a potential target for antimicrobial development. Based on these observations, we developed stable reporter plasmids that use gene complementation of SL1344_3500 to prevent plasmid loss during long-term in vivo studies.
DOI: 10.1128/jb.00054-25
Language: English
Subfield: Genetics
Open Access status: diamond
OpenAlex ID: W4413502001
FWCI:
Cites: 53
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Related to: 10