Antioxidant, Hypotensive, and Antidiabetic Breakthroughs: Bromelain Hydrolysis Unlocks Quinoa’s Peptide Potential - In Silico and In Vitro Approach
Year: 2025
Type: article
Abstract: High Resolution Image Download MS PowerPoint Slide This study integrates bioinformatics and experimental approaches to characterize bioactive peptides derived from quinoa 11S-globulin ( Chenopodium quinoa Willd) hydrolyzed in silico by stem bromelain (EC3.4.22.32). A total of 109 peptides were generated, of which 14 sequences with more than five amino acids were selected based on molecular docking and dynamics simulations against key metabolic targets (ACE-I, DPP-IV, α-glucosidase, and lipoxygenase). NIYQIS and QDQHQKIR demonstrated the highest binding affinities and hydrogen-bonding interactions, with ADMET predictions confirming their non-toxic and bioavailable profiles. In vitro, NIYQIS showed the strongest inhibitory activity against ACE-I (53%), DPP-IV (16.36%) and exhibited the highest antioxidant capacity (ORAC: 0.75 μM TE/μM peptide). Conversely, QDQHQKIR demonstrated the highest α-amylase inhibition (18.43%) and Cu 2+ chelation (40.4%), supporting its role in carbohydrate metabolism and metal-ion homeostasis. Overall, NIYQIS emerged as the most promising peptide, highlighting the potential of quinoa-derived peptides as functional ingredients to mitigate oxidative stress and metabolic disorders.
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: hybrid
Estimated APC paid: $4,500
Funders: Ministerio de Ciencia, Innovación y Universidades, University of Leeds, Consejo Nacional de Ciencia y Tecnología, CYTED Ciencia y Tecnología para el Desarrollo, Universidad Autónoma de Sinaloa, Directorate for Biological Sciences, Biotechnology and Biological Sciences Research Council, European Regional Development Fund, Agencia Estatal de Investigación
OpenAlex ID: W4413554402