Work

Comparison of the Synergistic Effects of Black Tea and Microwave with Gentamicin and Microwave on Acanthamoeba cyst Mortality In vitro

Year: 2025

Type: article

Abstract: Objective: poses a significant challenge, prompting the need for alternative treatments. This research aimed to explore the combined treatment of chemical or phytomedicines and microwaves radiation. Methods: strain was cultivated on non-nutrient agar. Black tea extracts were prepared using the maceration method. Final concentrations of 0.75 mg/mL and 0.375 mg/mL of gentamicin and tea, respectively, were used in this study. The samples were divided into 12 groups based on drug incubation time and repeated radiation exposure, either before or after incubation. The effects of combining gentamicin and black tea extracts with microwave exposure were then evaluated on the parasite. Results: was significantly higher in the combined treatment groups compared to gentamicin, black tea, or microwave radiation alone (p<0.0001-p<0.04). It seems that the microwave radiation led to an increasing trend in growth inhibition within 72 hours. Conclusion: cysts by gentamicin and black tea extracts. This effect was more significant on the irradiated cysts incubated with gentamicin and also depended on the increase in incubation time and the repetition of radiation.

Source: Turkish Journal of Parasitology

DOI: 10.4274/tpd.galenos.2025.50490

Authors: Zahra Eslamirad, Reza Hajihoseein, Yadollah Fathi, Homa Soleimani

Institution: Arak University of Medical Sciences

Language: English

Topic: Legionella and Acanthamoeba research

Subfield: Endocrinology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: diamond

Funder: Arak University of Medical Sciences