Year: 2025
Type: article
Abstract: In recent years, avidity has emerged as a critical parameter in antibody design, yet most current analytical instruments are limited to measuring affinity alone. This study aims to evaluate the capabilities and advantages of a novel surface plasmon resonance imaging instrument, CellVysion, designed to quantify cell-antibody avidity using a continuous antibody density gradient. A key feature of this approach is the identification of a "tipping point"-the specific ligand density, measured in µRIUs, at which cells remain bound to the sensor surface under defined shear flow conditions. In this paper, we present the technical principles and application of this method, demonstrating how avidity can be quantitatively assessed across different antibody-cell line combinations.
Source: Biosensors
DOI: 10.3390/bios15090559
Authors: Richard B. M. Schasfoort, Elise van Doorn, Jos van Weperen, Anouk Mentink, Ruchi Bansal
Institution: University of Twente
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $2,654
OpenAlex ID: W4413721497