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Identification of rotavirus in human and calf buffalo by using RT-PCR technique and rapid testing

Year: 2025

Type: article

Abstract: The molecular rotavirus, a significant zoonotic disease affecting calf water buffaloes and children within the same geographical region, was examined for the first time in Iraq. This virus is significant due to its activity, which induces gastroenteritis characterized by severe diarrhoea in all species, resulting in elevated mortality rates. Eighty-four fecal samples were collected from calves aged 4 to 6 months in the Abo-Gareeb area of Baghdad from December 2023 to April 2024. PCR identified positive results in 14 samples (16.6%) from a total of 84, with the peak infection rate occurring in January, comprising 8 samples (9.5%). In February and March, there were 4 samples (4.7%) and 2 samples (2.3%), respectively, along with 2 positive samples in December. Nevertheless, these statistics were documented in December. All samples underwent RT-PCR analysis using electrophoresis. Concurrently, 40 fecal bloody diarrhea swabs were obtained from children under the age of 2 belonging to buffalo breeders at Abo Gareb Hospital. The findings indicated that 8 of 40 samples tested positive for rotavirus (20%) using a Rapid Rotavirus test kit.

Source: International Journal of Veterinary Sciences and Animal Husbandry

Authors: Ibrahim Abdul-Hussein Swear Al-Zubaidy, Noor Muthanna Mahmood, Methaq Ghalib Abd AL-Rubaiey

Language: English

Topic: Viral gastroenteritis research and epidemiology

Subfield: Infectious Diseases

Field: Medicine

Domain: Health Sciences

SDGs: Good health and well-being, Zero hunger

Open Access status: diamond

OpenAlex ID: W4413727241

FWCI:

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