Work

Effect of Takadiastase on the Extraction of Biotin from Plant-Based Foods

Year: 2025

Type: article

Abstract: This study aimed to evaluate the extraction of biotin from plant-based foods using takadiastase in combination with autoclave-or ultrasound-assisted extraction.In cereals, vegetables, and mushrooms, autoclave-assisted enzymatic extraction obtained higher analytical values compared with autoclave-assisted extraction.However, in legumes and nuts, ultrasound-assisted enzymatic extraction obtained higher biotin content compared with either autoclave-or ultrasound-assisted extraction.Furthermore, method validation for the biotin analysis was performed to ensure the reliability of biotin extraction procedures from plant-based foods.The results demonstrated excellent linearity (R 2 =0.9999), accuracy (95.024%-102.507%recovery), and precision (1.158%-4.724%relative standard deviation), with limits of detection and quantification of 0.009 and 0.025 g/100 g, respectively.These findings could provide reliable data on the biotin content of commonly consumed plant-based foods in Korea.

Source: Preventive Nutrition and Food Science

DOI: 10.3746/pnf.2025.30.4.399

Authors: Naeun Kim, Yoonjeong Kim, Jihyeon Yu, Eunji Park, Youngmin Choi, Younghwa Kim

Institutions: Ministry of Food and Drug Safety, Kyungsung University, National Institute of Food and Drug Safety Evaluation, Rural Development Administration

Language: English

Topic: Biotin and Related Studies

Subfield: Cell Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Zero hunger

Open Access status: hybrid

Funder: Rural Development Administration