Growth Hormone Strongly Induces hSMN2 Promoter Driving Construct Gene Expression in Mammalian Cells
Year: 2025
Type: article
Abstract: What is already known on this topic?-Prolactin increases SMN expression and survival in a mouse model of severe spinal muscular atrophy via the STAT5 pathway.-STAT5 constitutive activation rescues defects in SMA.-HGH increases SMN expression and survival in severe SMA mouse model.-HGH treatment upregulates SMN protein in NT2 cells (Shown total SMN protein in the manuscript, it is not known how much SMN is coming from SMN1 gene or how much SMN protein is coming from SMN2).-GH majorly activates STAT5 activation. What this study adds?Nobody shows effect of GH on human SMN2 promoter using luciferase specific gene expression in mammalian cells.We did the first GH-SMN2 Promoter study in the world and our study shows GH specifically-strongly affect SMN2 promoter.Results showed that luciferase activity of the GH-treated pGL3-hSMN2 promoter 1 region increased 191.6-fold,GH-treated pGL3-hSMN2 promoter 2 region increased 348-fold and GH-treated pGL3-hSMN2 promoter 3 region increased 133-fold compared to GH-treated plasmid alone.These fold increases are too huge amount.GH may be used to increase SMN2 gene expression to treat SMA.
Authors: Dilara Yücedal, Ahmet Arman
Institution: Marmara University
Language: English
Subfield: Genetics
Field: Medicine
Domain: Health Sciences
Open Access status: gold
Estimated APC paid: $100
OpenAlex ID: W4413790101