Work

Protein profiling of diverse local and exotic Taramira (Eruca sativa Mill.) germplasm using SDS-PAGE analysis

Year: 2025

Type: article

Abstract: Genetic diversity study is important for the identification of elite genotypes.In this study, 150 local and exotic Taramira (Eruca sativa Mill.) genotypes were evaluated for total seed storage protein profiling using Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE) method.A 12.25% polyacrylamide gel was employed, revealing 14 distinct protein subunits across the genotypes.Among these, 11 bands were polymorphic while 3 were monomorphic, with molecular weights ranging from 10 to 180 kDa.The presence or absence of specific bands and variations in banding patterns reflected significant genetic variability among genotypes.The Dice similarity coefficient ranged from 74% to 100%, with the highest similarity observed in most accessions and the lowest between accessions 34910 and 34750.Cluster analysis grouped all genotypes into five major clusters.Overall, high polymorphism indicated substantial diversity among the studied genotypes.For more refined protein characterization, the use of 2-D gel electrophoresis is recommended.

Source: Pure and Applied Biology

DOI: 10.19045/bspab.2025.140076

Author: Nazma Bibi

Institution: Hazara University

Language: English

Topic: Genetic Mapping and Diversity in Plants and Animals

Subfield: Genetics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Zero hunger

Open Access status: gold

Estimated APC paid: $200