Work

Serial viral passage and reverse genetics protocol for identifying the antiviral drug targets of EV-D68 antivirals

Year: 2025

Type: article

Abstract: Identifying antiviral targets and mechanisms is crucial for drug discovery. Here, we present a protocol to select EV-D68 variants resistant to Jun6504 by combining serial passage and reverse genetics. We describe steps for viral serial passage, sequencing, and viral genome mutagenesis. We then detail procedures for producing and characterizing recombinant viruses. This protocol provides a robust strategy to identify targets from phenotypic screening hits.

Source: STAR Protocols

DOI: 10.1016/j.xpro.2025.104053

Authors: Haozhou Tan, Kan Li, Jun Wang

Institution: Rutgers, The State University of New Jersey

Language: English

Topic: Respiratory viral infections research

Subfield: Epidemiology

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $1,550