Work

Increased Expression of Bacterial Cellulose Synthase Genes in Komagataeibacter Xylinus Exposed to a Rotating Magnetic Field

Year: 2025

Type: article

Abstract: The objective of this study was to investigate the alterations in the expression of bacterial cellulose synthase (bcs) genes inKomagataeibacter xylinus, dependent on the exposure duration and specific parameters of a rotating magnetic field (RMF).K. xylinus cells were subjected to an RMF at frequencies of 5 and 50 Hz for durations ranging from 12 to 72 h. Gene expression was assessed using quantitative polymerase chain reaction (qPCR). The findings demonstrated that RMF exposure significantly enhanced BC production efficiency. Specifically, the average yields of wet and dry BC following RMF treatment increased by 28% and 18%, respectively, compared to the control. These improvements correlated with upregulated bcs gene expression, which showed statistically significant changes at both frequencies and all time points. The strongest effects occurred at 5 Hz. In conclusion, RMFs represent a promising approach to modulate gene expression in bacteria, offering substantial biotechnological potential, particularly for enhancing BC production.

Source: Biomacromolecules

DOI: 10.1021/acs.biomac.5c00653

Authors: Anna Zywicka, Aleksandra Dunisławska, Karol Fijalkowski

Institutions: West Pomeranian University of Technology in Szczecin, Bydgoszcz University of Science and Technology

Language: English

Topic: Advanced Cellulose Research Studies

Subfield: Biomaterials

Field: Materials Science

Domain: Physical Sciences

Open Access status: hybrid

Estimated APC paid: $4,500

Funder: Narodowe Centrum Nauki