Application of BSA-modified silica for solid phase extraction of progesterone from river water samples
Year: 2025
Type: article
Abstract: This study reports the development and application of bovine serum albumin (BSA)-modified silica gels as selective and durable sorbents for the solid-phase extraction (SPE) of progesterone from river water samples. One of the sorbents was used for SPE pre-concentration followed by progesterone quantification in a sample collected from the Danube River. Standard addition method followed by HPLC-DAD detection enabled the quantification of progesterone at a concentration of 143.0 ± 4.0 ng/L. At elevated progesterone concentrations (1.0 to 2.0×10-7 M), recoveries approached 100%, demonstrating high efficiency of the developed extraction sorbents. The overall method recovery ranged from 91.4 to 101.0%, confirming the method’s accuracy and robustness. Furthermore, under optimized SPE conditions, developed sorbents were highly selective towards progesterone compared to other reproductive hormones. The sorbents exhibited excellent long-term stability, with no significant change in binding efficiency observed over four years (variation lower than 3%), maintaining consistent recoveries of 94–96%. Newly developed BSA-modified silica sorbents represent a promising tool for trace analysis s of steroid hormones, as well as for their routine environmental monitoring.
Source: Talanta Open
Language: English
Subfield: Analytical Chemistry
Field: Chemistry
Domain: Physical Sciences
Open Access status: gold
Estimated APC paid: $2,190
OpenAlex ID: W4413821514