Work

Development of a 96-Well Plate Dithiothreitol Method for the Colorimetric Determination of Nickel Ions in Water Samples

Year: 2025

Type: article

Abstract: A new, simple, and accurate assay was developed for the colorimetric determination of nickel ions in 96-well plates. The proposed method utilized the color change immediately visible even to the naked eye during the reaction of Ni(II) ions with dithiothreitol molecules (DTT). The intensity of the color produced by the formation of the complex between nickel and dithiothreitol is linearly proportional to the concentration of the metal ions in an alkaline environment, at room temperature, and the detection was performed using a mobile phone as the detector. The proposed method had a good linear response between 0.05 and 0.75 mmol L−1 and a detection limit of 0.13 mmol L−1 and was successfully applied for the determination of Ni(II) in bottled and surface water samples.

Source: Sensors

DOI: 10.3390/s25175361

Authors: George N. Nikas, Maria Tarara, George Z. Tsogas

Institution: Aristotle University of Thessaloniki

Language: English

Topic: Analytical chemistry methods development

Subfield: Analytical Chemistry

Field: Chemistry

Domain: Physical Sciences

SDG: Clean water and sanitation

Open Access status: gold

Estimated APC paid: $3,137