Synaptic pruning following NMDAR-dependent LTD preferentially affects isolated synapses
Year: 2025
Type: article
Abstract: Long-term depression (LTD) is an activity-dependent decrease in synaptic strength. This state initiates either a re-potentiation or a loss, aka pruning, of the synapse within hours to days following its induction. However, the precise relationship between LTD and synaptic pruning remains elusive. Using various imaging techniques and electrophysiology on neuronal cell culture, we reveal a direct relationship between LTD and synaptic pruning. All synapses that exhibit NMDAR-dependent LTD are pruned unless counteracted by input-specific activity. Additionally, we identify the post-synaptic ion channels pivotal for sensing pre-synaptic activity. Finally, we find that neighboring synapses displaying coordinated activity act as mutual safeguards against pruning. These distinctive properties mean that LTD-dependent synaptic pruning has a major impact on synapses that do not contribute to responses involved in network activity.
Source: iScience
Language: English
Subfield: Cellular and Molecular Neuroscience
Field: Neuroscience
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $3,240
Funders: European Commission, Agence Nationale de la Recherche, Fondation pour la Recherche Médicale, Ministère de l'Education Nationale, de l'Enseignement Superieur et de la Recherche, Centre National de la Recherche Scientifique, European Research Council, Research Executive Agency, HORIZON EUROPE Marie Sklodowska-Curie Actions, HORIZON EUROPE Health, European Research Executive Agency
Awards: G1373765337, G2406513043, G2623647259, LocalDementia: Local protein turn-over at synapses in dementia, Tuning down synapses to remap the brain and adapt behavior, Regulation of assembly and disassembly of the postsynaptic density during synaptic plasticity and its effect on AMPAR trapping, Bordeaux Region Aquitaine Initiative for Neuroscience, Développment d'une infrastructure française distribuée coordonnée
OpenAlex ID: W4413826749