Work

Variovorax arabinosiphilus sp. nov., Variovorax flavidus sp. nov., Variovorax gracilis sp. nov., Variovorax brevis sp. nov., Variovorax jilinensis sp. nov., Variovorax davisae sp. nov., Variovorax saccharolyticus sp. nov. and Variovorax fucosicus sp. nov., isolated from ginseng rhizosphere

Year: 2025

Type: article

Abstract: Thirteen novel strains – J2L1-78T, J2P1-59T, J22R24T, J22R133T, J22P168T, J22P271T, J22R187T, J22R193T, J2L1-63, J2R1-6, J22G21, J22G47, and J31P216 – were isolated from the rhizosphere of 20-year-old ginseng in Jilin Province, P.R. China. These strains were Gram-stain-negative, aerobic and rod-shaped. Phylogenetic analysis based on the 16S rRNA gene sequences indicated their affiliation to the genus Variovorax, with 98.8–99.6% sequence similarity to Variovorax ginsengisoli Gsoil 3165T, Variovorax ureilyticus UCM-2T and Variovorax humicola UC38T. Phylogenomic analysis demonstrated their distinctiveness from closely related species. Average nucleotide identity (ANI) values among these strains confirm their representation of eight distinct species; additionally, ANI and digital DNA–DNA hybridization values between these strains and their closest relatives were below 92.5 and 54.1%, respectively. Eight representative strains contained C16 : 0, summed feature 3 (C16 : 1 ω7c and/or C16 : 1 ω6c) and C17 : 0 cyclo as major fatty acids. Based on phenotypic, phylogenetic and genotypic data, these 13 strains comprise 8 novel species within the genus Variovorax, for which we propose the following names: Variovorax arabinosiphilus sp. nov. (J2L1-78T=CGMCC 1.60704T=KACC 23365T), Variovorax flavidus sp. nov. (J2P1-59T=CGMCC 1.60707T=KACC 23366T), Variovorax gracilis sp. nov. (J22R24T=CGMCC 1.61001T=KACC 23367T), Variovorax brevis sp. nov. (J22R133T=CGMCC 1.61263T=KACC 23368T), Variovorax jilinensis sp. nov. (J22P168T=CGMCC 1.64555T=KACC 23372T), Variovorax davisae sp. nov. (J22P271T=CGMCC 1.64593T=KACC 23373T), Variovorax saccharolyticus sp. nov. (J22R187T=CGMCC 1.64629T=KACC 23374T) and Variovorax fucosicus sp. nov. (J22R193T=CGMCC 1.64631T=KACC 23375T).

Source: INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY

DOI: 10.1099/ijsem.0.006895

Authors: Yu-Hang Jiang, Ce-Ce Yin, Lei-Lei Yang, Yu-Hua Xin, Qing Liu, Jian Ye

Institutions: Chinese Academy of Sciences, Institute of Microbiology, University of Chinese Academy of Sciences, Guangxi University

Language: English

Topic: Genomics and Phylogenetic Studies

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: hybrid

Funder: National Natural Science Foundation of China

Award: G132271440