Work

Crude protoscolex antigens of echinococcus granulosus as serological markers for human hydatidosis detection

Year: 2025

Type: article

Abstract: Cystic echinococcosis (CE) or hydatidosis is a parasitic disease affecting both humans and animals. The present study aimed to evaluate the efficacy of the ELISA test in diagnosis of human hydatidosis using crude protoscolices antigens. Echinococcus granulosus protoscolices were isolated from the human liver hydatid cyst (local strain G1). Their crude antigen was prepared by the sonication technique. One hundred and eight sera samples were collected from patients with hydatid cyst (n = 21), individuals with other infections (n = 27) and sheep breeders (n = 60). These sera were evaluated using the ELISA assay. The predictable rate of sensitivity for the ELISA using the crude antigens was 100% depending on the cut-off. Additionally, the estimated values of specificity, positive and negative predictive values and accuracy were 97.4%; 87.3%; 100% and 97.8%, respectively. Crud E. granulosus protoscolices antigens are considered suitable candidates for the sero-diagnosis of human CE. To identify a single antigen and increase its effectiveness in serologic diagnostic testing, more research on these antigens is needed.

Source: Scientific Reports

DOI: 10.1038/s41598-025-17798-1

Authors: Dania Skhal, Abdul Qader Abbady, Hussain Chaban, Samar Al Nahhas

Institutions: Damascus University, Atomic Energy Commission of Syria, National University Hospital

Language: English

Topic: Parasitic infections in humans and animals

Subfield: Pathology and Forensic Medicine

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $2,690