Work

Non-invasive action potential recordings using printed electrolyte-gated polymer field-effect transistors

Year: 2025

Type: article

Abstract: Scalable and high-throughput platforms to non-invasively record the Action Potentials (APs) of excitable cells are highly demanded to accelerate disease diagnosis and drug discovery. AP recordings are typically achieved with the invasive and low-throughput patch clamp technique. Non-invasive alternatives like planar multielectrode arrays cannot record APs without membrane poration, preventing accurate measurements of disease states and drug effects. Here, we disclose reliable and non-invasive recording of APs with patch clamp-like quality from human stem cell-derived cardiomyocytes using an inkjet-printed polymer semiconductor in an Electrolyte-Gated Field-Effect Transistor configuration. High sensitivity is proven by the detection of drug-induced pro-arrhythmic membrane potential oscillations as early/delayed afterdepolarizations. The higher throughput potential of this platform could significantly enhance disease modelling, drug screening, safety pharmacology and the study of abiotic/biotic interfaces. Scalable platforms for non-invasive action potential recording are needed. Here, the authors present a reliable method to capture APs with patch-clamp-like fidelity from stem cell-derived cardiomyocytes using an Electrolyte-Gated Polymer Field-Effect Transistors.

Source: Nature Communications

DOI: 10.1038/s41467-025-63484-1