Efficient extraction of high molecular weight dsDNA from bacteriophage (QIAGEN silica-based membrane method) v1
Year: 2025
Type: preprint
Abstract: High molecular weight DNA can be efficiently extracted from dsDNA bacteriophages (phage) using a silica-based membrane method, such as the QIAGEN DNeasy Blood & Tissue Kit, with some modifications to significantly reduce the impact from host cell DNA contamination. Users can expect phage DNA concentrations of 2 to 100 ng/uL in 40 uL of elution buffer (median of approximately 15 ng/uL) if extracted from 450 uL of phage lysate containing > 1 x 109 PFU/mL. The concentration and yield of phage DNA is appropriate for long read whole genome sequencing, such as Oxford Nanopore Technology.
Authors: Mitchell G Hedges, Samuel Montgomery
Institution: The Kids Research Institute Australia
Language: English
Subfield: Ecology
Field: Environmental Science
Domain: Physical Sciences
Open Access status: gold
Funder: Medical Research Future Fund
OpenAlex ID: W4413873196