Year: 2025
Type: article
Abstract: Vegetative propagation through stem cuttings represents the primary mode of reproduction in Rosa species. While numerous studies have reported physiological factors affecting cutting rooting, the genes regulating the formation of adventitious roots in roses have not yet been fully explored and studied. In this study, we demonstrate that Rosa rugosa ‘Feng Hua’ exhibits an indirect rooting pattern, requiring callus formation prior to root primordium differentiation. Phytohormone profiling revealed exceptionally high concentrations of auxin precursors, particularly tryptophan (Trp), in both callus and root tissues. Therefore, we identified and analyzed the members of the YUCCA family, which are the key rate-limiting enzymes in the tryptophan-dependent IAA biosynthesis pathway. A total of 11 RrYUCs family genes were identified, with RT-qPCR analysis showing that RrYUC10 was highly expressed in callus and root tissues. Functional studies confirmed its critical role in adventitious root formation: virus-induced gene silencing (VIGS) of RrYUC10 significantly inhibited AR development, whereas its overexpression enhanced rooting. Our findings have provided a molecular theoretical basis for the rooting of cuttings in roses.
Source: Horticulturae
Authors: Mengjuan Bai, Yu Xi, Junqing Xue, Xiangfeng Xu, Mengmeng Xu, Liguo Feng
Institution: Yangzhou University
Language: English
Subfield: Plant Science
Domain: Life Sciences
SDG: Zero hunger
Open Access status: gold
Estimated APC paid: $2,654
Awards: G3036474686, G5959493889
OpenAlex ID: W4413880832