Generation of a PHF19 knockout human embryonic stem cell line by CRISPR/Cas9 system
Year: 2025
Type: article
Abstract: PHD finger protein 19 (PHF19) is a polycomb protein that promoted cardiac hypertrophy via epigenetic targeting SIRT2. To determine the role of PHF19 in myocardial hypertrophy, we established a large fragment knockout model of PHF19 gene in human embryonic stem cells (hESCs-H7) using the CRISPR/Cas9 system based on a vector. This PHF19-KO cell line has a normal karyotype, classical human pluripotent stem cell morphology, strong pluripotency, and significantly reduced PHF19 gene expression, which will become a useful tool for further in-depth research on the pathogenesis of PHF19 gene deficiency induced myocardial hypertrophy.
Source: Stem Cell Research
Authors: Yuqing Ran, Jinghua Ruan, Yijia Wang, Xing Feng, Pingping Tan, Yuting Guan, Xiaoling Guo
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $2,360
OpenAlex ID: W4413882015