Work

Isolation and molecular characterization of bacteriophages isolated from lake water and their application in foods against Bacillus cereus

Year: 2025

Type: article

Abstract: OBJECTIVES: Bacteriophages are viruses that specifically target and kill bacteria. Bacteriophages are considered safe for humans, making them suitable for use in food applications Therefore, this study was conducted to isolate, characterize, and apply bacteriophages from the environment to control food spoilage bacteria. RESULTS: PFU/mL). Both bacteriophages were polyvalent, targeting not only B. cereus, but also lysing enteropathogenic Escherichia coli (EPEC) and enterotoxigenic Escherichia coli (ETEC) with different efficiencies. Bacteriophage BC-VP and BC-AJ miMOI were determined to be 0.01 and 1, respectively. Bacteriophage BC-VP morphology was analyzed using Transmission Electron Microscope (TEM) and categorized as Myoviridae family from the order of Caudovirales. Both bacteriophages showed significant bacterial reduction when applied to artificially contaminated cooked rice and pasteurized milk. The reduction number was higher in pasteurized milk at 28℃ for BC-VP (93%) and BC-AJ (90%). Molecular characterizations were performed and showed that BC-VP was 96.88% a virulent bacteriophage. Also, no sign of antibiotic resistance gene in BC-VP and genes related to lytic cycle such as putative tail lysin and tail fiber, were annotated.

Source: BMC Research Notes

DOI: 10.1186/s13104-025-07436-4

Authors: Denny Rizkinata, Vania Clarissa Kusnadi, Diana Elizabeth Waturangi, Adi Yulandi

Institution: Atma Jaya Catholic University of Indonesia

Language: English

Topic: Bacteriophages and microbial interactions

Subfield: Ecology

Field: Environmental Science

Domain: Physical Sciences

SDGs: Zero hunger, Good health and well-being

Open Access status: gold

Estimated APC paid: $1,690