Decellularized porcine urinary bladder matrix as a dynamic biomaterial scaffold for myocardial cell adhesion
Year: 2025
Type: article
Abstract: • A decellularized porcine bladder matrix (UBM) was developed as a biocompatible scaffold for myocardial tissue engineering. • A dynamic perfusion culture system was employed to enhance cardiomyocyte adhesion. • The UBM scaffold supports calcium ion release, indicating potential bioactivity for cardiac regeneration. • The platform supports the physiological behavior of myocardial cells and offers significant potential for future applications in cardiac tissue repair and drug screening systems. Porcine urinary bladder matrix (UBM) is a promising extracellular matrix-derived scaffold due to its excellent biocompatibility and structural integrity. Tissue engineering approaches for cardiac repair require scaffolds that promote myocardial cell adhesion and physiological function. This study investigates the potential of UBM as a dynamic culture scaffold for myocardial cell adhesion and activity. UBM scaffolds were prepared by decellularizing porcine bladder tissue to remove cellular components while preserving the extracellular matrix. Cardiomyocytes were seeded onto the UBM scaffold and cultured under dynamic conditions using a custom-designed perfusion system to simulate physiological flow. Calcium ion activity was monitored to assess the functionality of myocardial cells. The decellularized UBM scaffold demonstrated high compatibility and supported adequate adhesion of myocardial cells under dynamic culture conditions. Although cardiomyocytes remained viable and adhered well to the scaffold, the calcium assay used in this study measured extracellular calcium levels in the supernatant and does not reflect intracellular calcium flux or contractile activity. Nonetheless, the sustained release of calcium ions from the UBM scaffold suggests bioactivity that may contribute to supporting cardiac tissue development.
Authors: Po-Shun Hsu, Ming-Fa Hsieh, Ming-You Shie, Wen-Ching Hsieh, Chun-Fan Lung, Yen-Hong Lin, Chao-Ling Yao, Chien-Sung Tsai, Yi-Chiung Hsu
Institutions: Tri-Service General Hospital, National Defense Medical Center, Chung Yuan Christian University, Asia University, China Medical University, China Medical University Hospital, National Central University, National Cheng Kung University, Ministry of National Defense, Cathay General Hospital
Language: English
Subfield: Surgery
Field: Medicine
Domain: Health Sciences
Open Access status: hybrid
Estimated APC paid: $2,650
Awards: The Application of Decellularized Porcine Bladder Matrix Scaffold in Cardiovascular Regenerative Medicine, G34000913, Exploring the Impact of Ventricular Assist Device Implantation on Myogenin Expression and Mitochondrial Metabolism in Human Failing Myocardial Cells, G4631010213, G4638784675, Engineered Dynamic Lung Models for Testing Personalized Therapies for Macro-Scale Lung Regeneration( III ), Deep-Learning-Based Analysis Identifies Exosome Mediated Diagnosis Signatures for Ards-Induced Lung Injury and Fibrosis.( II ), G7604718524
OpenAlex ID: W4413899763